HISAT samtools HTSeq ...
HISAT
wget ftp://ftp.ccb.jhu.edu/pub/infphilo/hisat2/downloads/hisat2-2.1.0-source.zipsamtools
unzip hisat2-2.1.0-source.zip
cd hisat2-2.1.0
make # 在此之前要安裝g++和gcc,用sudo apt install 安裝
rm -f *.h *.cpp
wget https://github.com/samtools/samtools/releases/download/1.5/samtools-1.5.tar.bz2HTSeq
tar xvfj samtools-1.5.tar.bz2
cd samtools-1.5
./configure # 根據configure檢測,安裝系統依賴的模塊,直到沒有報錯
make
sudo make install
# 安裝依賴
sudo apt-get install python-pip
pip install --upgrade pip
sudo apt-get install build-essential python2.7-dev python-numpy python-matplotlib python-pysam python-htseq
pip install HTSeq